Figure 5.
T cell responses induced by heterologous prime-boost vaccinations in outbred mice target nearly the entire 5GHPV3 immunogen. CD1 mice (10/group) were primed intramuscularly with either DNA-5GHPV3 (100 µg) or ChAdOx1-5GHPV3 (1 × 108 IU) and then boosted two weeks later with MVA-5GHPV3 (1 × 106 pfu). PBMC responses were determined by IFN-γ Elispot assays at two weeks post-prime and two and three weeks post-boost after stimulation with peptides spanning the entire immunogen sequence, pooled according to protein source. (A) Summed response to all pools and (B) responses to each peptide pool are shown. (C) Frequencies of 5GHPV3-specific CD8+ T cells in splenocytes were analysed three weeks post-boost by ICS in mice vaccinated with CM or DM. Unpaired t test, *p ≤ 0.05. (D) Mapping of responses to each segment in 5GHPV3 was performed on splenocytes from 10 CD1 mice vaccinated with CM at two weeks post-boost. Horizontal lines show mean with standard deviation.