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. 2019 Dec 10;9:18763. doi: 10.1038/s41598-019-55409-y

Figure 2.

Figure 2

Validation of DEGs with qRT-PCR. hBMECs induced by Neisseria (NM) or MafA (MafA). Black bars - logFC values from RNA-seq, white bars - logFC calculated from qRT-PCR. *Pearson correlation coefficient (PCC) r = 0.9966; P-value < 0.01; ** PCC r = 0.9959, P-value < 0.01. Please note that, standard deviation (SD) is not shown here as the expression level (log² of ΔΔCT) of DEGs in qRT-PCR was calculated based on average CT values of triplicates (the SD of CT were ranged between 0.005 to 0.28).