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. 2019 Dec 10;8(12):73. doi: 10.1038/s41389-019-0182-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b qRT-PCR and western blots indicate that cells overexpressing MACC1-AS1 increased the expression of PTN and c-Myc. **P < 0.01 as determined by Student’s t test. c, d qRT-PCR indicate that levels of PTN and c-Myc mRNAs were slightly changed when overexpressing MACC1-AS1 lacking a putative miRNA-384 or miRNA-145 binding site, respectively. n.s: not significant. e HEK-293T cells were transfected with miRNA-384 or miRNA-145 siRNA alone (red colored) or co-transfected with siRNAs and vectors expressing MACC1-AS1. qRT-PCR indicating that the miR-384-5p-mediated PTN or miR-145-3p-mediated c-Myc mRNA reduction could be partially rescued by overexpression of MACC1-AS1. **P < 0.01, *P < 0.05 as determined by one-way ANOVA assays. f MTT assays demonstrate that cells expressing mutant MACC1-AS1 without a miR-384-5p or miR-145-3p binding site reduced cell proliferation, in contrast to the cells expressing MACC1-AS1. *P < 0.05.