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. 2019 Dec 10;8(12):73. doi: 10.1038/s41389-019-0182-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, b qRT-PCR was performed to assess the levels of MCL-1 and PKM-1 mRNAs in MACC1-AS1-expressing MDA-MB-231 cells and in cells expressing siRNA against MACC1-AS1. **P < 0.01 and *P < 0.05 as determined by Student’s t test. c MDA-MB-231 cells transfected with constructs expressing MS2, MACC1-AS1-MS2 or MACC1-AS1(m)-MS2 for 72 h were subjected to MS2 pulldown assays. Representative western blots indicate that MACC1-AS1(m) was unable to associate with PTBP1. d Cells transfected as in (c) were subjected to immunoprecipitation with PTBP1 antibody or control IgG. Precipitated PTBP1 is shown by western blots. GAPDH was used as a control. e Levels of MACC1-AS1 and MACC1-AS1(m) in the PTBP1 precipitates were measured by qRT-PCR. MACC1-AS1(m) was not able to co-precipitate with PTBP1, when the binding site for PTBP1 was mutated. **P < 0.01 as determined by Student’s t test. f, g Levels of MCL-1 and PKM-1 mRNAs in the PTBP1 immunoprecipitates as in (d) were analyzed by qRT-PCR. Binding of MCL-1 and PKM-1 mRNAs to PTBP1 was significantly reduced in cells overexpressing MACC1-AS1. **P < 0.01 and *P < 0.05 as determined by Student’s t test.