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. 2019 Dec 10;10(12):945. doi: 10.1038/s41419-019-2176-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a A biotin-miRNA assay was utilized to verify the interaction between miR-516a-5p and circ_0001955, *P < 0.05. b Immunoprecipitation of AGO2 was performed in HCC cells to examine the interaction between miR-516a-5p and circ_0001955, *P < 0.05. c The sequence of wild-type (wt) and mutant (mut) miR-516a-5p. d Biotin-labeled miR-516a-5p (biotin-miR-516a-5p) and its negative control (biotin-miR-516a-5p mut) were transfected into HCC cells, and the streptavidin-captured circ_0001955 and GAPDH were detected by qRT-PCR, *P < 0.05. e AGO2 was cotransfected into HCC cells with miR-NC, miR-516a-5p and miR-516a-5p mut followed by detection of circ_0001955 and GAPDH, *P < 0.05.