Figure 2.

Loss of caz function impacts neuromuscular ability in Gem3 mutant flies. Left panel: In a heterozygous caz deficient background, brought about by the caz¹ null mutant, Gem3^BART flies develop flight defects at a late stage during adulthood. Hence, motor deficits become obvious only on day 35 post-eclosion. Middle panel: A greater reduction in Caz levels induced by a moderately-expressing RNAi transgene (caz-RNAi [Kellogg]) induced flight defects at an earlier stage (day 15 post-eclosion) and an increase in severity was observed with age. Right panel: A more pronounced age-dependent progressive decline in flight capacity can be brought about by knockdown mediated by a stronger RNAi transgene (caz-RNAi [Oriel]). Data presented are the mean ± S.E.M. of at least 4 independent experiments, and, for each time point measured, n ≥ 60 per genotype. Symbols indicate the sex of the genotype assessed: ♂ = males, and ♀ = females. Significance as tested by two-way ANOVA, followed by Bonferroni’s post hoc tests is indicated by the exact p-value.