Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 10;9:18666. doi: 10.1038/s41598-019-53508-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Overexpression of hTDP-43 or hFUS/caz^P398L suppresses NMJ expansion in Gem3 mutant flies. (A) Representative images of NMJs innervating ventral longitudinal muscles 6 and 7 in third instar larvae stained with post-synaptic anti-DLG antibody (scale bar = 10 μm). Visual inspection reveals that compared to wild-type, muscle-directed expression of Gem3^BART induces an overgrown NMJ morphology that is restored on overexpression of hTDP-43^WT, hFUS^WT and, to a lesser degree, caz^P398L. An overgrowth phenotype is also obvious on Glos knockdown (applied singularly or combined with Gem3^BART) or in flies expressing caz^P398L. These observations were confirmed upon quantification of NMJ area (B), number of branches per NMJ (C) and number of boutons within a single NMJ (D). In (B–D) data presented are the mean ± S.E.M. and n ≥ 18 per genotype. Equal number of male and female larvae were assessed. Significance as tested by the unpaired t-test is indicated by the exact p-value, shown either in blue (comparison to wild-type larvae) or black (comparison to Gem3^BART larvae).