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. 2019 Dec 10;11:1759091419886212. doi: 10.1177/1759091419886212

Figure 1.

Figure 1.

Atrophy in BACHD lumbar motoneurons. Representative images of motoneurons from lumbar spinal cord sections stained with ChAT from 12-month-old WT (a) and BACHD (b) animals. Scale bar: 50 µm. Fluorescence images of putative motoneurons stained with caspase-3 in WT (c) and BACHD (d—white arrows). Nuclei were stained with 4′,6-diamidino-2-phenylindole. Insert: putative motoneurons positive for caspase-3 in BACHD. Scale bar: 50 µm. (e) and (g) Quantification of ChAT- and OPN-positive motoneurons profiles in WT and BACHD lumbar spinal cords (∼150 neurons analyzed per genotype). Feret diameter for CHAT (f) and for OPN (h) (unpaired Student’s t test; *p < .05; n = 3 animals per genotype). Ultrastructure images showing a motoneuron with more lipofuscin granules (red arrows) in BACHD (j) compared with WT (i). (k and l) Representative images normal and vacuolated mitochondria in WT and BACHD, respectively. Scale bar: 500 nm. (m) Graphical quantification of motoneurons stained positively for caspase-3 in WT and BACHD (∼150 neurons analyzed per genotype; unpaired Student’s t test; **p < .002; n = 3 animals per genotype). N: Quantification of the number of lipofuscin granules/area in WT and BACHD motoneurons (total from 30 motoneurons per genotype; unpaired Student’s t test; p = .4; n = 3 animals per genotype). All results described here are from n = 3 individual animals per genotype and were expressed as mean ± SD. ChAT = choline acetyltransferase; WT = wild type.