Fig. 2.

Labeled miltefosine (MT-EtBDP) uptake evaluated by confocal microscopy. Parasites were adhered to poly-lysine coated plates and MF was added. Uptake was monitored during 8 min and fluorescence intensity over time was calculated. Uptake of MT-EtBDP (green) by L. (V.) braziliensis isolates LTCP 16012 (A) and LTCP 19446 (B) at the time of MT-EtBDP addition and after 3:30 and 7:39 min. The complete video is available as a supplementary file. (C) Measured fluorescence of each parasite in the focal field was plotted for area under the curve (AUC) analysis. Black lines represent the mean and gray shade represents the standard deviation of fluorescence intensity measured for each isolate population (LTCP 16012 n = 17 and LTCP, 19446 n = 11). Small dotted line represents the mean and SD of fluorescence units measured for background in different regions of the plate.