Fig. 3.

Residual fluorescence inside L. braziliensis isolates LTCP 16012 and LTCP 19446 was assessed by flow cytometry. After initial uptake determination, fluorescence associated with cell bodies was measured after 1 h, 2 h, 24 h and 48 h. Fluorescence intensity at each point time was normalized by the initial uptake of MT-EtBDP since these isolates showed differences in drug uptake. Each point represents mean and SD of three technical replicates and the graph show a representative experiment of three.