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. 2019 Aug 9;27(12):2182–2194. doi: 10.1016/j.ymthe.2019.07.020

Figure 2.

Figure 2

SMC1 Forms Intrachromosomal Looping at the ROR Locus

(A) Schematic diagram of the ROR lncRNA and the position of primers used for ChIP analyses. L1 through L6, primer names; arrows, transcriptional direction. (B) The interaction between SMC1 and ROR lncRNA. ChIP assay demonstrating that SMC1 binds the ROR promoter (−891 bp) and the upstream fragment (−3.8 kb). IgG is used as a negative control. Sites X, Y, and Z are ChIP detection sites. (C) The qPCR-ChIP assay showing the interaction between SMC1 and the ROR lncRNA. ChIP sites on the chromosome are listed at the bottom. Immunoglobulin G (IgG) is the negative control. All of the data are presented as the mean ± SD. *p < 0.05 compared with the negative control (fibroblast). (D) SMC1 interacted directly with MED12 as detected by co-immunoprecipitation. Cohesin (SMC1) is detected by western blot after AGS, HT29, and MUM2B immunoprecipitation. (E) ChIP assay detecting the interaction between MED12 and the ROR lncRNA. Sites X, Y, and Z are ChIP detection sites. M, marker.