Figure 6.

Enhancer Deletion Inhibits Tumor Growth In Vitro and In Vivo

(A) CCK8 assays show tumor cell growth after enhancer deletion. Cell growth was obviously restrained at day 3 in enhancer-deleted MUM2B cells. The absorbance values were detected at 24, 48, and 72 h, and the control was arbitrarily set at 100% on day 1. *p < 0.05, **p < 0.01 compared with mock. Scale bars, 500 μm (left; original magnification ×4), 100 μm (right; original magnification ×10). (B–D) Plate clone formation assays (B) and soft agar assays (C and D) demonstrate that the tumor formation ability was significantly reduced in enhancer-deleted MUM2B cells. *p < 0.05 compared with the mock. (E and F) Suppressive effects on tumor volume (F) and weight (G) in mice. *p < 0.05, **p < 0.01 compared with mock. (G) Mice were sacrificed, and tumors were isolated 20 days after inoculation. The growth of enhancer-deleted MUM2B cells was compared with empty vector-transfected cells. **p < 0.01 compared with mock. (H) Real-time PCR showing that ROR lncRNA expression was significantly decreased in the enhancer-deleted group. **p < 0.01 compared with mock.