Figure 1.

Biodistribution of rAAV9-EGFP in CNS

(A) IP and i.c.v. injections into E14–E15 mouse embryos. (B) IVIS imaging results at P1 and P14. The higher level of EGFP fluorescent signal was seen in the brain of pups that were administered i.c.v. injections from posterior view. In the anterior view of P1 and P14 pups; the liver of pups that were administered IP injections showed high GFP expression among internal organs. The level of EGFP expression was detected in both pups that received i.c.v. injections and those that received IP injections, specifically in intrascapular (posterior view) and inguinal (anterior view) regions known as BAT places. (C and D) Representative images of (C) cervical, thoracic, and lumbar spinal cord and (D) the brain’s coronal sections show a higher EGFP expression in the CNS regions of the mice receiving i.c.v. injections at P30. 4× objective. Scale bar, 500 μm.