Figure 4.

Survival Rate and Body Weight of Treated and Untreated SMA Mice

(A) The number of injected embryos and survival to birth by AAV vector. Injected SMA embryos had a lower survival rate than that of healthy embryos in all three groups. It was close to that of PBS-injected ones, which demonstrated that this increased mortality is not dependent on vector itself. (B) The lifespan of prenatally treated mice in each group was shown on a Kaplan-Meier plot (n = 10). The lifespan of treated animals in both study groups was significantly longer than that of the untreated subjects. Mice treated with scAAV9-SMN had a longer lifespan compared with ssAAV9-SMN. (C) Body weight of treated and untreated mice by age (n = 5). Although the body weight of SMA-treated pups increased slowly with age, it was still significantly less than that of healthy wild-type pups. (D) Treated and untreated pups at P14. This image clearly demonstrates the smaller body sizes of treated mice when compared with healthy wild-type pups. p < 0.001, by Student’s unpaired two-tailed t test. Data shown indicate mean ± SD.