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. 2019 Nov 14;105(6):1148–1167. doi: 10.1016/j.ajhg.2019.10.007

Figure 4.

Figure 4

Characterization of Sperm Motility and Fertilization Potential of Ttc29−/− L5 and L7 Mutant Mice

(A) Assessment of sperm kinematic movement of Ttc29−/− L5 and L7 mutant mice by CASA, indicating reduced curvilinear velocity (VCL) and beat/cross frequency (BCF) as compared with controls (WT) while the amplitude of lateral head displacement (ALH) was found unaffected. Data are represented as the mean ± SEM p value < 0.05 (∗); p value < 0.01 (∗∗); p value < 0.001 (∗∗∗); non-significant (ns).

(B) In vitro fertilization assays with capacitated sperm from Ttc29−/− L5 and L7 epididymes, showing reduced fertilization rates and fertilization index when inseminated with cumulus-intact oocytes and Zona-free oocytes, respectively, as compared with control sperm (WT). The total number of analyzed oocytes is indicated above the histogram of each mouse genotype. Data are represented as the mean ± SEM p value < 0.01 (∗∗); p value < 0.001 (∗∗∗); p value < 0.0001 (∗∗∗∗). Each experiment was repeated three times.

(C) In vivo breeding assays of Ttc29−/− L5 and L7 mutant mice with wild-type females, showing a reduced number of pups per litter (left) and of litters per female (right) over a breeding period of 124 days, as compared to control males (WT). The total numbers of litters analyzed and the number of female breeders used for each genotype is indicated at the top of the histograms (left and right, respectively). Data are represented as the mean ± SEM p value < 0.05 (∗); p value < 0.01 (∗∗); p value < 0.001 (∗∗∗).