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. 2019 Nov 14;105(6):1148–1167. doi: 10.1016/j.ajhg.2019.10.007

Figure 5.

Figure 5

Localization of TTC29 Orthologous Protein, TbTTC29, in T. brucei Flagellar Axoneme

(A) Representative picture of detergent-extracted parental cells immunolabelling, with anti-PFR (red) that labels the para-axonemal paraflagellar rod structure and anti-TY1 (green) antibodies, as a negative experimental control.

(B) Representative picture of detergent-extracted TbTTC29::TY1 cell line immunolabelling with anti-PFR (red) and anti-TY1 (green) antibodies, showing axonemal localization of the endogenous recombinant TbTTC29::TY1 at both the old flagellum (OF) and the new flagellum (NF).

(C) Representative picture detergent-extracted TbTTC29::TY1 cell line immunolabelling with anti-FTZC labeling the transition zone (red) and anti-TY1 (green) antibodies, showing localization of TbTTC29::TY1 from the transition zone to the distal tip of the axoneme. The mitochondrial genome and the nuclei are stained with DAPI. Scale bars represent 5 μm in main figures and 1 μm in zoom insets. OF and NF indicated the old flagellum and the new flagellum, respectively.