Figure 1.

Effects of Hypoxia on lncRNA-MEG3 Expression

(A) Mice were exposed to hypoxia (10% FiO₂) for indicated times, and lncRNA-MEG3 expression was detected by real-time PCR in isolated PAs (n = 6). (B) FISH analysis of lncRNA-MEG3 (red) and colocalization with αSMA (green) in PAs from 21-day hypoxia-exposed mice. (C) Expression of lncRNA-MEG3 was detected in PASMCs of iPAH patients and in PASMCs from human and mouse (n = 6). (D) FISH showing the distribution lncRNA-MEG3 (red, CY3 staining) in the nucleus (blue, stained with 4,6-diamidino-2-phenylindole) and cytoplasm. (E) Quantification of FISH fluorescence signals of lncRNA-MEG3 in the nuclear and cytoplasmic fractions. U6 and 18S RNA were used as controls for localization of the nucleus and cytoplasm, respectively. The graph represents the means ± SEM from >50 cells from three independent experiments. (F) Isolation of cytoplasmic and nuclear fractions followed by real-time PCR (n = 4). Data represent means ± SEM from indicated independent experiments. Student’s t test (for two means) or one-way ANOVA followed by Dunnett’s test (for >2 means). *p < 0.05, **p < 0.01.