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. 2019 Aug 22;27(12):2166–2181. doi: 10.1016/j.ymthe.2019.07.022

Figure 4.

Figure 4

Overexpression of MEG3 Induces PASMC Proliferation, Cell-Cycle Progression, and Cellular Migration

(A) One segment of lncRNA-MEG3 (mouse transcript varies 1, nt 1563–3207, 1644 bp) was amplified by real-time PCR, and then inserted into lentiviral vectors. Expression of lncRNA-MEG3 in mPASMCs after transfection with different doses of plasmid. (B) Cell viability determined by MTT. (C) BrdU incorporation. (D) PCNA expression detected by western blot. (E) Cell-cycle progression detected by flow cytometry. (F) Cyclin expression and (G) CDK expression detected by western blot. (F) Cellular migration. Data represent means ± SEM from four independent experiments. One-way ANOVA followed by Dunnett’s test was used. *p < 0.05, **p < 0.01.