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. 2019 Nov 14;105(6):1168–1181. doi: 10.1016/j.ajhg.2019.10.010

Figure 4.

Figure 4

Affected TTC30A and IFT52 Immunostaining in the Spermatozoa from Men Harboring TTC29 Mutations

(A) Sperm cells from a normal control and from men harboring TTC29 mutations were stained with anti-TTC30A (green, a component of IFT complex B) and anti-α-tubulin (red) antibodies. DNA was counterstained with DAPI as a nuclei marker. In spermatozoa from the fertile control, TTC30A staining appears to be located mainly in the basal body and lightly decorates the sperm flagellum. In the sperm cells from men harboring TTC29 mutations, TTC30A staining is strongly reduced or totally absent. Scale bars: 5 μm.

(B) Sperm cells from a normal control and from men harboring TTC29 mutations were stained with anti- IFT52 (red, a key component of the IFT-B complex) and anti-α-tubulin (green) antibodies. DNA was counterstained with DAPI as a nuclei marker. IF52 staining was found mainly at the base of the flagellum and faintly along cilia in control spermatozoa but revealed weak staining and only aggregated at the base of sperm flagella in men harboring TTC29 mutations. Scale bars: 5 μm.