Figure 5.
TEM Cross-Sections of Spermatozoa from Ttc29mut/mut Male Mice Reveal Multiple Structural Axonemal Defects
(A) Cross-sections of the mid-piece of sperm flagellum in a WT male mouse. The axoneme is composed of nine peripheral microtubules doublets and a central pair of singlet microtubules, surrounded by nine outer dense fibers and the mitochondrial sheath.
(B) Cross-sections of the principal piece of sperm flagellum from a WT male mouse. Typical ‘‘9 + 2’’ microtubule structure was observed and surrounded by outer dense fibers and the fibrous sheath.
(C–F) Various axonemal anomalies can be observed, including the lack of the central pair of microtubules (C, D) or randomly oriented outer dense fibers (E, F).
Abbreviations: CP, central pair of microtubules (red arrows); MT, peripheral microtubule doublet (yellow arrows); ODF, outer dense fiber (blue arrows); WT, wild type. Scale bars: 200 nm.