Figure 6.

Impaired Fertilization Capability by Loss of Ttc29 Function Could Be Rescued by Intracytoplasmic Sperm Injection

(A) Representative two-cell embryos and blastocysts from in vitro fertilization. Scale bar, 200 μm. The two-cell rates and blastocyst rates were counted after caudal epididymal sperm fertilized oocytes that were collected from superovulated wild-type (WT) females. Here, the blastocyst rates were shown as the percentage of blastocysts out of total oocytes. The WT, Ttc29^+/mut, and Ttc29^mut/mut groups consisted of three, three, and eight male mice, respectively. A total of 1,305 embryos were counted. Data are represented as means ± SEM; ^∗∗∗p < 0.001. Abbreviations: n.s., not significant; WT, wild type.

(B) Representative two-cell embryos and blastocysts from intracytoplasmic sperm injection. Scale bar, 200 μm. The two-cell rates and blastocyst rates were counted after sperm heads of Ttc29-mutated male mice were injected into oocytes that were collected from superovulated wild-type (WT) females. Here, the blastocyst rates were shown as the percentage of blastocysts out of total injected oocytes. The WT, Ttc29^+/mut, and Ttc29^mut/mut groups consisted of three, three, and six male mice, respectively. A total of 759 embryos were counted. Data are represented as means ± SEM. Abbreviations: n.s., not significant; WT, wild type.