Table 3.
Clinical Outcomes of ICSI Cycles Using Spermatozoa of TTC29-Mutated, DNAH1-Mutated Men with MMAF and Controls
| - | Group A | Group B | Group C | Comparisons of Three Groups | A versus B | A versus C | B versus C | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| - | TTC29-Mutated MMAF | DNAH1-Mutated MMAF | OAT as Controls | F/H Value | p | Test Statistic | p | Test Statistic | p | Test Statistic | p |
| No. of couples | 3 | 11 | 301 | NA | NA | NA | NA | NA | NA | NA | NA |
| Mean male age (years) | 31.7 ± 6.7 | 32.7 ± 4.7 | 30.1 ± 4.9 | 1.718 | 0.181 | N/A | 1.000 | N/A | 1.000 | N/A | 0.231 |
| Mean female age (years) | 27.3 ± 3.2 | 28.3 ± 4.2 | 28.4 ± 4.2 | 2.500 | 0.084 | N/A | 0.491 | N/A | 1.000 | N/A | 0.088 |
| No. of ICSI cycles | 3 | 11 | 301 | NA | NA | NA | NA | NA | NA | NA | NA |
| No. of oocytes injected | 11 (7,24) | 10 (6, 20) | 11(1, 34) | 0.362 | 0.834 | −0.472 | 1.000 | −0.413 | 1.000 | −0.435 | 1.000 |
| Fertilization rate (%) | 71.4 (63.6, 91.7) | 63.6 (40.0, 86.0) | 83.3 (12.5, 100.0) (3219/4077) | 8.700 | 0.013 | −0.707 | 1.000 | −0.726 | 1.000 | −2.872 | 0.012∗ |
| Cleavage rate (%) | 100.0 (80.0, 100.0) | 100.0 (70.0, 100.0) | 100.0 (66.7, 100.0) (3188/3219) | 4.455 | 0.108 | −0.091 | 1.000 | −0.966 | 1.000 | −1.909 | 0.168 |
| 8-cell formation rate (%) | 40.9 (0, 85.7) | 57.1(0, 100.0) | 70.6 (10.5, 100.0) | 2.859 | 0.239 | −1.101 | 0.873 | −1.349 | 0.531 | −1.031 | 0.909 |
| Blastocyst formation rate (%) | 31.8 (0, 57.1) | 53.5 (0, 100.0) | 60.0 (0, 100.0) | 4.979 | 0.083 | −1.018 | 1.000 | −1.882 | 0.180 | −1.232 | 0.654 |
| No. of transfer cycles | 2 | 9 | 301 | NA | NA | NA | NA | NA | NA | NA | NA |
| Number of embryos transferred per cycle | 1 (0, 2) | 2 (0,3) | 2 (1, 2) | 1.993 | 0.369 | −0.742 | 1.000 | −1.368 | 0.513 | −0.421 | 1.000 |
| Implantation rate (%) | 66.7 (2/3) | 31.3 (5/16) | 47.9 (229/478) | NA | NA | N/A | 0.592b | 0.005 | 0.945a | 1.723 | 0.190 |
| Clinical pregnancy rate per transfer cycle (%) | 100.0 (2/2) | 55.6 (5/9) | 57.1 (172/301) | NA | NA | 0.136 | 0.712a | 0.254 | 0.614a | 0.061 | 0.805a |
| Miscarriage rate (%) | 0 (0/2) | 20.0 (1/5) | 11.6 (20/172) | NA | NA | NA | NA | N/A | NA | N/A | 0.945b |
Symbols: NA, not available data or not being compared; N/A, not applicable, statistical analysis was performed by Fisher’s exact test and thus without the Chi-square value; ∗, the corrected inspection level, α of 0.0167 was used to analyze pairwise comparisons among three groups for categorical data.
Annotations: A versus B, group A compared with group B; A versus C, group A compared with group C; B versus C, group B compared with group C; OAT, oligoasthenoteratozoospermia; F, statistics of ANOVA; H, statistics of Kruskal-Wallis test.
Statistical analyses were performed by an independent statistician blinded to clinical outcomes. Descriptive statistics were expressed using mean ± standard deviation for normally distributed variables. ANOVA was used for measurement data. The Chi-square test or Fisher’s exact test was performed to analyze categorical data. The non-normal distributed variables were shown with median (min, max) and analyzed with independent-samples Kruskal-Wallis test. All the tests were performed using a two-sided test of difference where the inspection level α of 0.05 and a difference with p < 0.05 were considered statistically significant. Pairwise comparisons among the three groups were corrected by Bonferroni method for measurement data.
Yate’s corrected Chi-square test
Fisher’s exact test