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. 2019 Aug 22;27(12):2100–2110. doi: 10.1016/j.ymthe.2019.08.008

Figure 2.

Figure 2

Physicochemical Characterization of FM@PFC/siRNA Nanoemulsions

(A) Hydrodynamic size distribution of FM@PFC. (B) TEM of FM@PFC. (C) Zeta potential of the formulations (polymer/siRNA w/w ratio 2). (D) Cytotoxicity of PEI, M, FM, and FM@PFC in L929 cells. (E) Agarose gel retardation assay at varying M/siRNA w/w ratios. (F) Cell association of polyplexes (M/siRNA and FM/siRNA) and nanoemulsions (FM@PFC/siRNA) analyzed by flow cytometry in 4T1.Luc cells after 4 h incubation with the formulations prepared with FAM-siRNA at w/w ratio 2 (siRNA 100 nM). Data are presented as the mean ± SD with unpaired t test, n ≥ 3, **p < 0.01, ***p < 0.001. (G) Extent of endosomal escape at 1–6 h.