Figure 6.

Mechanism of Action of FM@PFC/siSTAT3 Nanoemulsions

(A) Western blot analysis for Bcl-2, pERK, E-cadherin, p-STAT3, and STAT3 in 4T1.Luc cells treated with different formulations prepared at polymer/siRNA w/w ratio 2 (siSTAT3 100 nM). (B) Analysis of immunohistochemistry staining of p-STAT3 in lung metastasis. (C) Relative expression of VEGF, MMP9, and CD31 in lung metastasis. (D) Ki67 positive% in the lung metastasis. (E) CD8⁺ T cells infiltration in lung metastasis. Relative (F) PD-L1, (G) IFN-γ, (H) IL-12, and (I) Foxp3 expression in lung metastasis. Semiquantitation of the staining was made with ImagePro Plus. Data are presented as the mean ± SD, n = 3, unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001.