Fig. 3.

Nitroimidazole (DNDI-0690), benzoxaborole (DNDI-6148), and aminopyrazole (DNDI-1047) significantly reduce the bioluminescence signal (parasite load) at the infection site in BALB/c mice infected with luciferase-expressing L. major parasites. Mice received 50 mg/kg DNDI-6148, 25 mg/kg DNDI-0690, and 25 mg/kg DNDI-1047. All drugs were given PO, BID, for 10 consecutive days except for DNDI-6148 which was given for 5 consecutive days. The positive control group was treated with 25 mg/kg AmBisome® which was given IP, QD for 10 consecutive days. Vehicle control groups (VC 1, VC 2, and VC 3) consisted of 2% ETOH, 5% dextrose (aq); PEG 400; and 0.5% w/v methylcellulose, 5% v/v Tween 80/ddH₂O respectively, which were the solvents used to dissolve DNDI-6148, DNDI-0690, and DNDI-1047 respectively. All vehicle control groups were treated PO, QD for 10 consecutive days, except for the VC1, which was chosen to be administered BID instead of QD. Each point represents mean ± standard error for the bioluminescence signal. One-way ANOVA was used to analyse differences between the positive, negative, and experimental groups. A p-value < 0.05 was considered statistically significant (*: p < 0.05).