Fig. 1.

Percent of penetration and retention of AmB from Lip-AmB 0.1, 0.2 and 0.4% across the mouse skin after 24 h. Franz cell diffusion studies were carried out with jacketed Franz cells contain PBS as the receiver medium at 37 °C, and samples were drawn at 1-h intervals for up to 24 h (A). At the end of the experiment, the formulation that remained on the top of the mouse skin was collected and assayed for detection of the amount of AmB. The permeation rate of AmB in regard to time (B). The percent formulation retention was calculated by considering the percentage of the drug released and the remaining amount on the skin at the end of the 24 h experiment. Values are means ± standard deviations (n = 3).