Fig. 4.

Changes in kidneys pathology. The renal toxicity evaluation by pathological examination of kidneys after topical application of liposomal AmB 0.4% twice a day for 30 days. One of the kidneys from each mouse was removed and fixed in 10% neutral-buffered formalin solution, and then was embedded in paraffin. Embedded kidneys were cut at 3 μm thickness and stained with hematoxylin and eosin (HE). The images were prepared at A) 10 × and B) 40 × magnification. The kidneys were normal and no adverse effect was found in the examined kidneys.