Fig. 6.

Splenic and lesion parasite burden in BALB/c mice treated with liposomal AmB formulations. Spleens and infected lesions were aseptically removed and homogenized in 1 mL RPMI-FCS at weeks 8 and 12 after infection. The homogenates were diluted and placed in 96-well microtiter plates containing solid layer of rabbit blood agar in triplicate and incubated at 25 ± 1 °C for 7 days. The motile promastigotes were checked using an inverted microscope (CETI, UK). The splenic parasite load in group of mice treated with Lip-AmB 0.4% was significantly lower than groups of mice treated with PBS or empty liposomes at week 12 post infection (***, p<0.001) (Fig. 6A). Load of parasite in the lesion of mice treated with Lip-AmB 0.4% was significantly lower than all other groups at day 8 and 12 post infection (***, p<0.001) (Fig. 6B).