Table 1.

Particle size, zeta potential, AmB concentrations, MIC^a (against L. major promastigotes) and ED₅₀ (against L. major amastigotes in J774 A.1 macrophage) of topical liposomal AmB formulations.

Liposome formulations	Z-average (nm)	Zeta potential (mv) ± SD	AmB concentration (mg/g) ± SD	MICa(μg/mL)	ED50(μg/mL) (lower and upper 95% limit)
Control empty liposomes	106.3 ± 2.1	−32.9 ± 4.59	–	Inactive	Inactive
Lip-AmB 0.1%	119.2 ± 14.4	−32.7 ± 6.13	1.17 ± 0.0058	0.625	0.151 (0.0523–0.434)
Lip-AmB 0.2%	116.7 ± 9.9	−36.4 ± 7.98	2.36 ± 0.0058	0.625	0.151 (0.085–0.267)
Lip-AmB 0.4%	113.5 ± 10.4	−36.5 ± 4.2	4.11 ± 1.53	0.625	0.0856 (0.0147–0.5)
Fungizone	–	–	–	0.625	0.063 (0.027–0.146)

The values are means ± standard deviations (n = 3).

^aMinimum inhibitory concentrations.