FIG 10.
Inhibition of the integrated stress response impedes UL148-mediated ER remodeling. (A and B) Tet-on ARPE-19 epithelial cells that inducibly express UL148 fused to green florescent protein (GFP), i148GFP, were induced for transgene expression using 100 ng/ml doxycycline (dox) in the presence of DMSO carrier, ISRIB (200 nM), or GSK2606414 (1.1 μM) and monitored by anti-UL148 Western blotting for expression of UL148-GFP over a series of time points (h posttreatment with dox [hpt]). (C to E) Live cell imaging of UL148-GFP expression patterns in the presence of ISRIB (200 nM), GSK2606414 (1.1 μM), or DMSO vehicle (0.01%). Scale bars, 50 μm or 10 μm (for inset panels at upper left of each image, which are magnified 2.4× relative to the main image). See also Movies S3 to S5 in the supplemental material.