Figure S5.

Oxygen Tension Functions as an Important Switch between Injury and Regeneration, Related to Figure 6

(A) Hopx expression was assayed following indicated time points after exposure to 2% O2 or re-exposure to 21% O2 by transient 4-OH tamoxifen labeling (24hrs prior) in ALI monolayers derived from Hopx^CreER/Rosa^Td mice.

(B-C) H&E stained sections of ALId21 monolayers treated with indicated concentrations of HIF activators including VH298, DMOG and DFO for 48hrs (B). Immunoblots for the Hif1α protein in cell lysates following 8hrs of the indicated HIF activator treatment (C).

(D) H&E, Ki67 and UEA1 stained sections from ALId21 monolayers treated with ER stress inducers including BFA and TM for 48hrs.

(E) Scheme for assessing the effect of low oxygen tension on the regenerative capacity of ALId0 cells. After 7 days of initial submersion, monolayer cells were subject to ALI in either 2% O₂ or 21% O₂ condition for 21 days.

(F) ALId21 monolayers treated with either 2% O₂ or 21% O₂ were examined for cell morphology (left panels), Cd44/Ki67 double staining (mid panels) and UEA1 staining (right panels).

Bars: (A, B, D and F) 25 μm. Histological and immuno-fluorescent images are representative of at least three samples examined.