Figure 3.

M-L Screen Gene Knockout Phenotypes at the Liver Stage Show a Bias toward Genes with Predicted Metabolic Function

(A) Ranked, normalized log₂FC values at each stage transition and for all genes with data. SG-B2 data were corrected for blood stage fitness, and an apparent increase in some mutants (right end of distribution) results from some overcompensation for slow growth. Error bars show standard deviations.

(B) Pie charts show the distribution of phenotypes (see C for legend).

(C) SG-B2 phenotypes shown separately for genes that at the blood stage (Bushell et al., 2017) grow slowly (yellow) or are indistinguishable from wild type (green).

(D) Plots showing the blood-stage (inner ring) and liver-stage (SG-B2, outer ring) phenotypes for genes pertaining to specific metabolic subsystems (upper row) or GO biological processes (lower row). Liver-stage phenotypes for genes are clustered according to their corresponding blood-stage phenotype. The association of genes to metabolic subsystems is based on iPbe. GO biological process data, where available, are displayed in Table S2.