Figure S5.

Phenotypic Analysis of Gene Knockout Mutant Parasite Lines Associated with the FAE Pathway, Related to Figure 6

(A) Graph showing growth rate of ΔKCRv1 blood stage parasites in relation to control parasites, as shown as progression of parasitemia at successive days after injection of blood stage parasites. (B) Graph showing oocyst numbers (relative to control; set to 100%) at day 7 post infection for ΔELO-A, ΔKCRv1, ΔKCRv2, ΔKCRv2 (xWT) and ΔCBR parasites. Error bars indicate standard deviation. The results were statistically evaluated by a one-way analysis of variance (ANOVA) test with Dunnet’s multiple comparisons (^∗∗p < 0.01). Boxes shown below the graph indicate the presence (green) or absence (red) of sporozoites from the salivary glands for each knockout strain between days 18 and 21 post-infection. (C) Graph showing oocyst numbers in the ΔKCRv1 parasite strain relative to the control on days 4, 6, 8, 10 and 13 post-infection. Error bars display standard deviation. (D) Table displaying sexual and mosquito stage phenotypic data for ΔKCRv1 parasites; gametocyte conversion rate, exflagellation rate, female: male ratio, zygote to ookinete conversion rate and percentage of ookinetes showing abnormal morphology. Data is shown in relation to normal ranges for such phenotypic assessments. (E) Graphs showing relative blood stage parasitemia for mice injected with ΔELO-A parasites (relative light units by luciferase assay) and parasitemia for mice injected with ΔKCRv2 (x WT) and ΔCBR parasites (based on FACS). Data from all mice (control and KOs) are shown for two independent experiments and lines are drawn for the relative light unit level or parasitemia considered as being the point at which a mouse has become positive.