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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Cell Rep. 2019 Jun 25;27(13):3760–3769.e4. doi: 10.1016/j.celrep.2019.05.100

Figure 4. Prp19 Is Inefficiently Recruited to ICGs in the Absence of Eaf3.

Figure 4.

(A) Western blot analysis of Prp19-HA protein levels in wild-type and eaf3Δ cells. Pgk1 is a loading control.

(B) Occupancy of Prp19-HA on ICGs HPC2 (left) and RAD14 (right) relative to a non-transcribed region (NTR) on Chr. V in wild-type (light gray bars) and eaf3Δ (medium gray bars).

(C) Occupancy of Prp19-HA on ICG ECM33 relative to a NTR on Chr. V. in wild-type and eaf3Δ cells. Bars represent the average of at least 3 biological replicates. Error bars represent the SEM. p values were determined by 2-tailed unpaired t test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.