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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Cell Rep. 2019 Jun 25;27(13):3760–3769.e4. doi: 10.1016/j.celrep.2019.05.100

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-H3K36me3 Abcam Cat# ab9050; RRID:AB_306966
Anti-Histone H3 Abcam Cat# ab1791; RRID:AB_302613
Anti-HA (12CA5) Roche Cat# 11583816001; RRID:AB_514505
Anti-Myc (9E10) Roche Cat# 11667149001; RRID:AB_390912
Anti-RNAPII S2P Abcam Cat# ab5095; RRID:AB_304749
Chemicals, Peptides, and Recombinant Proteins
Bovine Serum Albumin Rockland Cat# BSA-50
Non-fat Dry Milk Genesee Scientific Cat# 20-241
Agencourt AMPure XP Beckman Coulter Cat# A63880
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Roche Cat# 4693159001
Formaldehyde Fisher Scientific Cat# BP531-500
iTaq Universal SYBR® Green Supermix Bio-Rad Cat# 172-5121
GammaBind G Sepharose GE Healthcare Cat# 17-0885-01
Pierce Anti-HA Magnetic Beads ThermoFisher Scientific Cat# 88836
Maxima Reverse Transcriptase ThermoFisher Scientific Cat# EP0742
RiboLock RNase Inhibitor ThermoFisher Scientific Cat# EO0382
RNase A ThermoFisher Scientific Cat# AM2270
Proteinase K Roche Cat# 03115828001
DNase I recombinant Roche Cat# 4716728001
Glass Beads BioSpec Cat# 11079105
Protein A Dynabeads ThermoFisher Scientific Cat# 10001D
HiBind DNA columns VWR Cat# 95043-212
UltraPure Ethidium Bromide ThermoFisher Scientific Cat# 15585011
Critical Commercial Assays
TruSeq Stranded mRNA Library Preparation Kit Illumina Cat# RS-122-2101
Ribo-Zero Gold rRNA Removal Kit (Yeast) Illumina Cat# MRZY1324
Ovation Ultralow DR Kit NuGEN Cat# 0344
Deposited Data
RNA-seq and ChiP-seq data This study GEO: GSE120051
Experimental Models: Organisms/Strains
Yeast strains, see Table S1 This study N/A
Oligonucleotides
Primers for PCR, RT-PCR, and qPCR, see Table S3 This study N/A
Recombinant DNA
pFA6a-3HA-kanMX6 Longtine et al., 1998 N/A
pFA6a-3HA-HIS3MX6 Longtine et al., 1998 N/A
pFA6A-13Myc-kanMX6 Longtine et al., 1998 N/A
Software and Algorithms
Prism 7 GraphPad N/A
TopHat 2.0.14 Trapnell et al., 2009 https://ccb.jhu.edu/software/tophat/index.shtml
MACS2 Zhang et al., 2008 https://github.com/taoliu/MACS
SeqPlots 3.7 Stempor and Ahringer, 2016 https://bioconductor.org/packages/release/bioc/html/seqplots.html
Bowtie 0.12.8 Langmead et al., 2009 http://bowtie-bio.sourceforge.net/index.shtml
Microsoft Excel (Version 1812) Microsoft N/A
ImageJ NIH https://imagej.nih.gov/ij/