KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-H3K36me3 | Abcam | Cat# ab9050; RRID:AB_306966 |
| Anti-Histone H3 | Abcam | Cat# ab1791; RRID:AB_302613 |
| Anti-HA (12CA5) | Roche | Cat# 11583816001; RRID:AB_514505 |
| Anti-Myc (9E10) | Roche | Cat# 11667149001; RRID:AB_390912 |
| Anti-RNAPII S2P | Abcam | Cat# ab5095; RRID:AB_304749 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bovine Serum Albumin | Rockland | Cat# BSA-50 |
| Non-fat Dry Milk | Genesee Scientific | Cat# 20-241 |
| Agencourt AMPure XP | Beckman Coulter | Cat# A63880 |
| cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail | Roche | Cat# 4693159001 |
| Formaldehyde | Fisher Scientific | Cat# BP531-500 |
| iTaq Universal SYBR® Green Supermix | Bio-Rad | Cat# 172-5121 |
| GammaBind G Sepharose | GE Healthcare | Cat# 17-0885-01 |
| Pierce Anti-HA Magnetic Beads | ThermoFisher Scientific | Cat# 88836 |
| Maxima Reverse Transcriptase | ThermoFisher Scientific | Cat# EP0742 |
| RiboLock RNase Inhibitor | ThermoFisher Scientific | Cat# EO0382 |
| RNase A | ThermoFisher Scientific | Cat# AM2270 |
| Proteinase K | Roche | Cat# 03115828001 |
| DNase I recombinant | Roche | Cat# 4716728001 |
| Glass Beads | BioSpec | Cat# 11079105 |
| Protein A Dynabeads | ThermoFisher Scientific | Cat# 10001D |
| HiBind DNA columns | VWR | Cat# 95043-212 |
| UltraPure Ethidium Bromide | ThermoFisher Scientific | Cat# 15585011 |
| Critical Commercial Assays | ||
| TruSeq Stranded mRNA Library Preparation Kit | Illumina | Cat# RS-122-2101 |
| Ribo-Zero Gold rRNA Removal Kit (Yeast) | Illumina | Cat# MRZY1324 |
| Ovation Ultralow DR Kit | NuGEN | Cat# 0344 |
| Deposited Data | ||
| RNA-seq and ChiP-seq data | This study | GEO: GSE120051 |
| Experimental Models: Organisms/Strains | ||
| Yeast strains, see Table S1 | This study | N/A |
| Oligonucleotides | ||
| Primers for PCR, RT-PCR, and qPCR, see Table S3 | This study | N/A |
| Recombinant DNA | ||
| pFA6a-3HA-kanMX6 | Longtine et al., 1998 | N/A |
| pFA6a-3HA-HIS3MX6 | Longtine et al., 1998 | N/A |
| pFA6A-13Myc-kanMX6 | Longtine et al., 1998 | N/A |
| Software and Algorithms | ||
| Prism 7 | GraphPad | N/A |
| TopHat 2.0.14 | Trapnell et al., 2009 | https://ccb.jhu.edu/software/tophat/index.shtml |
| MACS2 | Zhang et al., 2008 | https://github.com/taoliu/MACS |
| SeqPlots 3.7 | Stempor and Ahringer, 2016 | https://bioconductor.org/packages/release/bioc/html/seqplots.html |
| Bowtie 0.12.8 | Langmead et al., 2009 | http://bowtie-bio.sourceforge.net/index.shtml |
| Microsoft Excel (Version 1812) | Microsoft | N/A |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |