Table 2. Between-group comparison of circulating miRNA expression.
| Storage condition | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| miRNAs, | immediately frozen at - 80°C | 24h RT | 24h 4°C | |||||||||
| fold change (P) | PPT vs K2EDTA | K2EDTA vs PPP | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P |
| hsa-miR-1 | 12.84 | 0.02 | 0.29 | 0.092 | 0.02 (0.027) |
0.12 (0.470) |
0.16 (0.699) |
0.032 | 0.02 (0.027) |
0.20 (0.470) |
0.11 (0.699) |
0.032 |
| hsa-miR-106a-5p | 1.36 | 1.78 | 2.41 | 0.051 | 0.60 (0.539) |
3.62 (0.539) |
0.17 (0.022) |
0.027 | 0.73 | 0.58 | 4.41 | 0.079 |
| hsa-miR-10b-5p | 4.36 | 1.00 | 4.36 | 1.000 | 323.51 (0.022) |
23.92 (0.539) |
13.52 (0.539) |
0.027 | 203.30 (0.022) |
35.11 (0.539) |
15.03 (0.539) |
0.027 |
| hsa-miR-122-5p | 0.80 | 0.37 | 0.30 | 0.061 | 3.31 (0.539) |
0.34 (0.539) |
9.71 (0.022) |
0.027 | 2.22 (0.539) |
0.33 (0.539) |
0.023 (0.022) |
0.027 |
| hsa-miR-125b-5p | 0.22 (0.539) |
169.01 (0.022) |
36.73 (0.539) |
0.027 | 1.00 | 21.01 | 0.05 | 0.063 | 0.02 | 0.03 | 0.52 | 0.064 |
| hsa-miR-1260a | 43.13 | 0.94 | 40.37 | 0.058 | 0.01 (0.022) |
0.42 (0.539) |
0.03 (0.539) |
0.027 | 0.01 (0.022) |
0.79 (0.539) |
0.58 (0.539) |
0.027 |
| hsa-miR-128-3p | 1.00 | 1.12 | 1.12 | 0.869 | 1.01 | 53.70 | 0.02 | 0.064 | 0.02 (0.028) |
0.71 (0.539) |
1.17 (0.539) |
0.027 |
| hsa-miR-140-5p | 41.24 | 1.00 | 41.24 | 0.063 | 1.00 | 32.90 | 0.03 | 0.063 | 1.10 (0.699) |
41.32 (0.027) |
36.22 (0.470) |
0.032 |
| hsa-miR-143-3p | 34.31 (0.539) |
0.01 (0.022) |
0.22 (0.539) |
0.027 | 1.38 | 28.73 | 0.05 | 0.055 | 0.01 | 0.98 | 0.25 | 0.067 |
| hsa-miR-144-3p | 0.17 (0.034) |
1.74 (0.890) |
0.29 (0.408) |
0.039 | 6.30 | 0.51 | 12.43 | 0.148 | 2.31 | 1.63 | 0.19 | 0.491 |
| hsa-miR-144-5p | 53.84 | 1.04 | 56.03 | 0.064 | 0.01 (0.034) |
0.45 (0.890) |
0.02 (0.408) |
0.039 | 1.62 (0.539) |
0.27 (0.539) |
76.36 (0.022) |
0.027 |
| hsa-miR-146b-5p | 32.94 (0.539) |
0.01 (0.022) |
0.26 (0.539) |
0.027 | 0.01 (0.022) |
0.49 (0.539) |
0.02 (0.539) |
0.027 | 0.01 | 0.83 | 0.63 | 0.061 |
| hsa-miR-152-3p | 0.47 (0.539) |
106.31 (0.022) |
50.45 (0.539) |
0.027 | 1.81 | 1.03 | 1.75 | 0.067 | 0.02 (0.408) |
2.70 (0.890) |
0.01 (0.034) |
0.039 |
| hsa-miR-155-5p | 56.78 (0.408) |
0.01 (0.034) |
0.41 (0.890) |
0.039 | 0.01 | 0.61 | 0.02 | 0.067 | 1.10 | 67.05 | 62.57 | 0.064 |
| hsa-miR-15a-5p | 1.97 (0.539) |
0.20 (0.022) |
0.39 (0.539) |
0.027 | 2.40 | 0.81 | 2.95 | 0.061 | 1.21 | 0.43 | 0.41 | 0.061 |
| hsa-miR-15b-3p | 63.95 | 1.00 | 63.95 | 0.063 | 0.02 (0.034) |
0.55 (0.890) |
0.03 (0.408) |
0.039 | 1.76 (0.539) |
0.54 (0.539) |
56.45 (0.022) |
0.027 |
| hsa-miR-16-2-3p | 45.83 (0.539) |
0.01 (0.022) |
0.26 (0.539) |
0.027 | 0.01 | 0.01 | 1.00 | 0.063 | 0.01 | 0.02 | 0.01 | 0.058 |
| hsa-miR-186-5p | 1.21 | 0.83 | 1.00 | 0.965 | 0.01 (0.022) |
0.35 (0.539) |
0.03 (0.539) |
0.027 | 0.58 | 1.21 | 21.01 | 0.634 |
| hsa-miR-191-5p | 1.94 (0.890) |
3.57 (0.408) |
6.94 (0.034) |
0.039 | 0.16 | 1.15 | 0.14 | 0.051 | 0.34 (0.890) |
1.67 (0.408) |
2.42 (0.034) |
0.039 |
| hsa-miR-194-5p | 28.90 (0.539) |
0.01 (0.022) |
0.16 (0.539) |
0.027 | 5.78 (0.539) |
0.39 (0.539) |
14.87 (0.022) |
0.027 | 2.63 (0.539) |
0.53 (0.539) |
0.18 (0.022) |
0.027 |
| hsa-miR-195-5p | 46.57 | 1.00 | 46.57 | 0.063 | 0.01 (0.022) |
0.54 (0.539) |
0.03 (0.539) |
0.027 | 0.01 (0.303) |
2.48 (0.990) |
0.34 (0.051) |
0.051 |
| hsa-miR-199a-5p | 0.46 (0.539) |
60.84 (0.022) |
28.07 (0.539) |
0.027 | 0.87 | 48.86 | 0.02 | 0.064 | 0.87 | 49.74 | 48.86 | 0.064 |
| Storage condition | ||||||||||||
| miRNAs, | immediately frozen at - 80°C | 24h RT | 24h 4°C | |||||||||
| fold change (P) | PPT vs K2EDTA | K2EDTA vs PPP | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P |
| hsa-miR-210-3p | 33.68 (0.539) |
0.005 (0.022) |
0.16 (0.539) |
0.027 | 1.00 | 1.17 | 0.85 | 0.723 | 0.02 | 0.02 | 0.02 | 0.058 |
| hsa-miR-2110 | 42.84 | 0.01 | 0.52 | 0.082 | 0.01 (0.034) |
0.40 (0.890) |
0.03 (0.408) |
0.039 | 0.01 (0.022) |
0.48 (0.539) |
0.44 (0.539) |
0.027 |
| hsa-miR-215-5p | 0.36 (0.303) |
0.87 (0.990) |
0.31 (0.051) |
0.051 | 1.16 | 33.39 | 0.03 | 0.064 | 3.25 (0.539) |
0.66 (0.539) |
93.70 (0.022) |
0.027 |
| hsa-miR-21-5p | 1.61 | 1.08 | 1.75 | 0.067 | 1.20 | 1.17 | 1.02 | 0.202 | 0.20 (0.539) |
1.64 (0.539) |
0.19 (0.022) |
0.027 |
| hsa-miR-223-3p | 1.53 (0.539) |
1.61 (0.539) |
2.48 (0.022) |
0.027 | 0.07 | 1.55 (0.539) |
0.04 (0.022) |
0.027 (0.539) |
0.15 | 0.95 | 3.42 | 0.067 |
| hsa-miR-24-3p | 1.54 (0.539) |
3.51 (0.539) |
5.39 (0.022) |
0.027 | 0.20 (0.303) |
1.12 (0.990) |
0.18 (0.051) |
0.051 | 0.66 (0.539) |
2.45 (0.539) |
3.67 (0.022) |
0.027 |
| hsa-miR-29c-3p | 55.34 (0.539) |
0.004 (0.022) |
0.21 (0.539) |
0.027 | 0.01 | 0.01 | 1.00 | 0.063 | 0.01 | 0.75 | 0.01 | 0.061 |
| hsa-miR-301a-3p | 0.36 (0.539) |
170.97 (0.022) |
61.12 (0.539) |
0.027 | 0.01 (0.034) |
0.71 (0.890) |
0.02 (0.408) |
0.039 | 0.02 (0.539) |
2.54 (0.539) |
1.13 (0.022) |
0.027 |
| hsa-miR-30a-5p | 34.37 | 1.07 | 36.71 | 0.058 | 358.44 (0.022) |
40.39 (0.539) |
8.87 (0.539) |
0.027 | 4.54 | 1.14 | 0.51 | 0.061 |
| hsa-miR-30e-3p | 38.32 (0.539) |
38.32 (0.022) |
0.33 (0.539) |
0.027 | 0.01 (0.034) |
0.46 (0.890) |
0.02 (0.408) |
0.039 | 0.02 | 1.75 | 0.72 | 0.061 |
| hsa-miR-32-5p | 0.02 (0.539) |
0.47 (0.539) |
0.01 (0.022) |
0.027 | 0.02 (0.022) |
0.53 (0.539) |
0.04 (0.539) |
0.027 | 1.10 | 6.99 | 28.49 | 0.107 |
| hsa-miR-331-3p | 0.01 (0.034) |
1.64 (0.890) |
0.01 (0.408) |
0.039 | 0.02 | 0.95 | 0.02 | 0.061 | 0.03 | 1.63 | 1.76 | 0.061 |
| hsa-miR-335-3p | 50.45 | 0.86 | 43.19 | 0.058 | 0.01 (0.022) |
0.32 (0.539) |
0.03 (0.539) |
0.027 | 0.02 | 1.36 | 0.87 | 0.067 |
| hsa-miR-339-5p | 0.58 (0.890) |
119.87 (0.034) |
69.93 (0.408) |
0.039 | 0.01 | 1.01 | 0.01 | 0.067 | 0.02 | 1.10 | 2.22 | 0.067 |
| hsa-miR-363-3p | 0.45 (0.539) |
138.59 (0.022) |
63.02 (0.539) |
0.027 | 4.91 | 0.71 | 6.96 | 0.061 | 1.44 | 0.64 | 0.21 | 0.252 |
| hsa-miR-374a-5p | 40.32 (0.470) |
1.29 (0.699) |
52.14 (0.027) |
0.032 | 0.01 (0.051) |
0.64 | 0.02 | 0.051 | 0.02 | 1.22 | 0.69 | 0.067 |
| hsa-miR-374b-5p | 1.22 | 49.60 | 60.46 | 0.061 | 0.03 (0.539) |
2.06 (0.539) |
0.01 (0.022) |
0.027 | 0.02 | 0.96 | 1.45 | 0.067 |
| hsa-miR-375 | 0.22 (0.539) |
195.35 (0.022) |
42.75 (0.539) |
0.027 | 0.01 (0.022) |
0.22 (0.539) |
0.03 (0.539) |
0.027 | 11.12 (0.539) |
0.11 (0.539) |
427.55 (0.028) |
0.027 |
| hsa-miR-378a-3p | 0.14 (0.539) |
0.51 (0.539) |
0.07 (0.022) |
0.027 | 4.73 (0.539) |
0.39 (0.539) |
12.24 (0.022) |
0.027 | 2.20 | 0.57 | 0.18 | 0.079 |
| hsa-miR-409-3p | 67.32 (0.027) |
0.89 (0.699) |
59.60 (0.470) |
0.032 | 0.01 | 0.43 | 0.01 | 0.061 | 0.02 | 1.42 | 1.33 | 0.067 |
| hsa-miR-421 | 31.58 | 1.00 | 31.58 | 0.063 | 0.01 (0.034) |
0.48 (0.890) |
0.03 (0.408) |
0.039 | 0.02 (0.022) |
0.64 (0.539) |
0.63 (0.539) |
0.027 |
| hsa-miR-424-5p | 0.01 | 126.48 | 1.00 | 0.063 | 291.39 (0.022) |
26.76 (0.539) |
10.89 (0.539) |
0.027 | 0.02 | 0.02 | 0.00 | 0.063 |
| Storage condition | ||||||||||||
| miRNAs, | immediately frozen at - 80°C | 24h RT | 24h 4°C | |||||||||
| fold change (P) | PPT vs K2EDTA | K2EDTA vs PPP | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P | PPP vs K2EDTA | PPT vs K2EDTA | PPT vs PPP | P |
| hsa-miR-425-5p | 91.97 (0.408) |
0.01 (0.034) |
0.50 (0.890) |
0.039 | 0.01 | 0.62 | 0.01 | 0.067 | 1.28 | 0.89 | 124.48 | 0.561 |
| hsa-miR-454-3p | 49.88 (0.027) |
0.76 (0.699) |
37.92 (0.470) |
0.032 | 0.01 (0.022) |
0.50 (0.539) |
0.03 (0.539) |
0.027 | 0.02 | 0.63 | 0.88 | 0.063 |
| hsa-miR-483-5p | 39.02 (0.539) |
0.04 (0.022) |
0.14 (0.539) |
0.027 | 2.29 (0.408) |
0.27 (0.890) |
8.59 (0.034) |
0.039 | 0.01 | 0.96 | 0.00 | 0.061 |
| hsa-miR-486-5p | 1.59 (0.539) |
0.23 (0.022) |
0.37 (0.539) |
0.027 | 1.79 (0.539) |
0.21 (0.539) |
8.35 (0.022) |
0.027 | 3.73 (0.408) |
0.81 (0.890) |
0.45 (0.034) |
0.039 |
| hsa-miR-501-3p | 1.28 | 0.78 | 1.00 | 0.723 | 0.01 | 0.01 | 0.84 | 0.064 | 0.02 (0.022) |
0.40 (0.539) |
0.02 (0.539) |
0.027 |
| hsa-miR-505-3p | 38.61 | 1.00 | 38.61 | 0.063 | 0.01 (0.022) |
0.21 (0.539) |
0.02 (0.539) |
0.027 | 0.01 | 0.01 | 0.38 | 0.055 |
| hsa-miR-629-5p | 0.20 | 0.72 | 0.15 | 0.55 | 5.01 (0.539) |
0.23 (0.539) |
21.97 (0.022) |
0.027 | 0.02 | 0.45 | 0.00 | 0.055 |
| hsa-miR-660-5p | 1.00 | 1.00 | 1.00 | 1.000 | 3.45 (0.539) |
0.02 (0.539) |
164.86 (0.022) |
0.027 | 0.02 (0.022) |
0.54 (0.539) |
0.00 (0.539) |
0.027 |
| hsa-miR-7-5p | 13.02 | 0.02 | 0.29 | 0.092 | 1.00 | 6.20 | 0.16 | 0.106 | 0.02 (0.027) |
0.15 (0.470) |
0.15 (0.699) |
0.032 |
| hsa-miR-766-3p | 0.52 (0.539) |
63.76 (0.022) |
33.10 (0.539) |
0.027 | 0.01 (0.034) |
0.78 (0.890) |
0.02 (0.408) |
0.039 | 0.02 (0.539) |
3.35 (0.539) |
1.45 (0.022) |
0.027 |
| hsa-miR-877-5p | 37.53 (0.539) |
0.012 (0.022) |
0.45 (0.539) |
0.027 | 0.74 | 33.42 | 0.02 | 0.063 | 0.01 (0.022) |
0.56 (0.539) |
0.38 (0.539) |
0.027 |
| hsa-miR-885-5p | 7.21 (0.699) |
0.01 (0.027) |
0.06 (0.470) |
0.032 | 1.00 | 6.18 | 0.16 | 0.106 | 0.02 | 0.59 | 0.14 | 0.129 |
| hsa-miR-93-3p | 78.27 | 0.01 | 0.77 | 0.061 | 0.01 (0.034) |
0.52 (0.890) |
0.03 (0.408) |
0.039 | 2.01 | 0.77 | 73.21 | 0.061 |
| hsa-miR-99a-5p | 1.15 | 0.87 | 1.00 | 0.723 | 0.01 (0.022) |
0.48 (0.539) |
0.02 (0.539) |
0.027 | 0.02 (0.051) |
0.79 (0.990) |
1.06 (0.303) |
0.051 |
| hsa-miR-99b-5p | 1.79 (0.890) |
50.77 (0.408) |
90.83 (0.034) |
0.039 | 0.01 | 1.37 | 0.01 | 0.067 | 0.03 (0.408) |
1.97 (0.890) |
3.22 (0.034) |
0.039 |
| Expressions for each miRNAs from K2EDTA, PPP and PPT samples and each storage condition were compared through Kruskal-Wallis test. P < 0.05 was considered statistically significant. Dunn’s multiple comparison test was performed for comparison between two storage conditions, only for significant Kruskal-Wallis test. Differences in miRNA expression between two conditions is reported as the ratio of miRNA expression of an “experimental” condition and a “reference” condition (fold change). Results are reported only for miRNAs with a statistically significant ≥ ± 5-fold change, in at least one comparison. K2EDTA - dipotassium ethylendiaminetetraacetate tubes. PPT – plasma preparation tubes. PPP- platelet-poor plasma. | ||||||||||||