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. 2019 Dec 10;20:963. doi: 10.1186/s12864-019-6359-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — PCR amplification of markers TTE1E-214 (a), TTE1E-3 (b), and TTE1E-58 (c). Lanes M marker, 1 CS, 2 wheat cultivar Shumai482, 3 wheat cultivar Shumai921, 4 diploid Thinopyrum elongatum, 5 tetraploid Thinopyrum elongatum, 6 8801 (T. durum-tetraploid Th. elongatum amphidiploid), 7 K17–841-1 (wheat–tetraploid Th. elongatum substitution line), 8 TDS2E(2A), 9 TDS3E(3D), 10 TDS4E(4D), 11 TDS5E(5D), 12 TDS6E(6D), 13 TDS7E(7D). Arrows show the diagnostic amplification products of tetraploid Th. elongatum 1E chromosome