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Sequences of wheat 1DS and 1DL SSR markers
| Marker | Primer sequence (5′-3′) | Annealing temperature (°C) | Arm location | Amplification size (bp) |
|---|---|---|---|---|
| wmc147 |
F: AGAACGAAAGAAGCGCGCTGAG R: ATGTGTTTCTTATCCTGCGGGC |
58 | 1DS | 154 |
| wmc222 |
F: AAAGGTGCGTTCATAGAAAATTAGA R:AGAGGTGTTTGAGACTAATTTGGTA |
54 | 1DS | 184 |
| gwm337 |
F: CCTCTTCCTCCCTCACTTAGC R: TGCTAACTGGCCTTTGCC |
59 | 1DL | 191 |
| Xcfd63 |
F: TCCTGAGGATGTTGAGGACC R: GAGAGAGGCGAAACATGGAC |
58 | 1DL | 282 |
F forward primer, R reverse primer
