Fig. 3.

LRF/ZBTB7A switches 3T3-L1 preadipocytes’ fate from cellular proliferation to terminal differentiation. Human LRF/ZBTB7A expression in 3T3-L1 preadipocytes promotes cell growth arrest at the stage of mitotic clonal expansion via inhibition of Sp1-dependent activation of cyclin A, cyclin-dependent kinase 2, and p107, while indirectly (via E2F-4 inhibition] activates PPARγ and aP2 transcription factors, known to be involved in terminal differentiation of 3T3-L1 cells into adipocytes (in red color are cellular processes inhibited and in green color are cellular processes activated by LRF/ZBTB7A expression). Abbreviations: aP2, adipocyte fatty acid-binding protein; E2F-4, E2F transcription factor 4; PPARγ, peroxisome proliferator-activated receptor gamma; Sp1, specificity protein 1