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. 2019 Dec 10;15:448. doi: 10.1186/s12917-019-2196-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Titers of anti-Enolase antibody isotypes of mice immunized with rEnolase in combination with 4 adjuvants. Two 50 μg doses of rSsEno adjuvanted in Quil-A®, Polygen™, Stimune® or Montanide™ ISA 50 V2 were administered to mice. A control group (non-immunized) received 100 μL of phosphate-buffered saline. Isotypes were detected in sera twelve days after the first (Dose 1) and the second (Dose 2) vaccination. Data are expressed as mean ± standard error of the mean. * (p < 0.05) indicates a significant difference between non-immunized and dose 1. # (p < 0.05) indicates a significant difference between dose 1 and dose 2 and a (p < 0.05) between treated groups (adjuvants) for the second immunization (Dose 2)