Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | General characteristics | Source/reference |
|---|---|---|
| Escherichia coli | ||
| TOP10 | F− mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| BL21 | F−ompT hsdSB (rB−, mB−) gal dcm rne131 (DE3) | Invitrogen |
| Streptococcus suis | ||
| 31533 | Virulent European serotype 2 ST1 strain isolated from pig with meningitis | (42) |
| ΔdppIV | Isogenic mutant strain derived from P1/7 strain. In frame deletion of dppIV | (20) |
| Group B Streptococcus | ||
| COH-1 | Capsular type III strain isolated from an infant with bacteremia | (40) |
| Plasmids | ||
| pET-32a | Apr, pBR322 ori, T7 promotor | Novagen |
| pBAD/thio | Apr, pUC ori, PBAD promotor | Invitrogen |
| pET-32aEnolase | pET-32a carrying eno gene for protein production | This work |
| pBAD/thioDPPIV | pBAD/thio-TOPO carrying dppIV gene for protein production | This work |