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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Cell Rep. 2019 Nov 12;29(7):1756–1766.e8. doi: 10.1016/j.celrep.2019.10.008

Figure 3. Longitudinal eOD-GT8 60-mer-Specific BGC and Non-BGC Cell Responses in Draining LNs after a Single IM or s.c. Immunization.

Figure 3.

(A) Flow cytometry identification of total eOD-GT8 60-mer-specific LN B cells before and after immunization (top and bottom samples pairs are from individual RMs). Gated on CD20+ B cells. Full gating in Figure S2.

(B) Frequency of total eOD-GT8 60-mer-specific B cells after IM or s.c. immunization (Mann-Whitney, one tailed).

(C) Flow cytometry identification of eOD-GT8 60-mer-specific plasmablast (KI67+BCL6−) or GC (KI67+BCL6+) B cells in the LN.

(D) Frequency of KI67+BCL6− plasmablasts among eOD-GT8 60-mer-specific B cells in the draining axillary LN over time.

(E) Frequency of BGC cells among total eOD-GT8 60-mer-specific B cells in the draining axillary LN over time (Mann-Whitney, two tailed).

(F) Representative flow cytometry gating of eOD-GT8 60-mer-specific B cells among BGC cells.

(G) Frequency of eOD-GT8 60-mer-specific BGC cells in the draining axillary LN over time (Mann-Whitney, two tailed).

(H) Total number of eOD-GT8 60-mer-specific BGC cells in the LN FNA sample of the draining axillary LN.

(I) Total number of eOD-GT8 60-mer-specific B cells in the LN FNA sample of the draining axillary LN.

*p < 0.05. Each point represents an individual LN FNA sample. n = 8, four LN FNAs per immunization condition at each time point.

See also Figures S2 and S3.