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. 2019 Dec 11;16:262. doi: 10.1186/s12974-019-1664-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Nef does not change the amount of total SMAD4 in astrocytes and neurons. a–d Total SMAD4 expression in astrocytes and neurons show no significant difference between Nef and GFP samples; neither when treated with SD208. e Representative immunoblot of total SMAD4 in astrocytes expressing Nef or GFP confirms total SMAD4 protein expression