Fig. 1.

Comparison of untransduced and hNIS-mGFP⁺ HLCs 2 days after transduction | (A) Scheme depicting the differentiation stages of iPSCs to HLCs (top) combined with micrographs showing the morphologies of both A1AT and CGT10 HLCs at the corresponding differentiation stages. Representative live cell flow cytometric analyses of differentiating HLCs on day 20 showing (B) GFP signals in transduced HLCs and (C) comparable surface marker expression of the hepatic progenitor marker EpCAM. mRNA expression analysis (by qRT-PCR) of an immature hepatocyte gene panel 2 days after transducing the differentiating cells: (D) EpCAM, (E) Hepatic nuclear factor 4α (HNF4A), (F) ɑ-fetoprotein (AFP), and (G) Cytochrome P450 Family 3A7 (CYP3A7). Control and transduced cells were always from the same differentiation batches and values normalised to corresponding control cells. N = 3 biological replicates (i.e. independent differentiations); error bars are SEM; two-tailed Student's t-test was applied (p > 0.05 for all).