Fig. 2.

Comparison of untransduced and hNIS-mGFP⁺ HLCs two weeks after transduction | (A) Immunofluorescence micrographs demonstrating hNIS-mGFP⁺cells mature following passage and co-express the gold standard markers for hepatic cells, albumin and HNF4A. Images representative of 3 independent differentiations. Scale bars 100 µm. (B) Secreted albumin concentrations are not significantly different between control and transduced batches. Similarly, no significant differences were detected for (C-E) marker gene expression by qRT-PCR (normalised to corresponding control batches). (F) Day 34 hNIS expression by qRT-PCR relative to expression at day 20 demonstrating expression is stable (G-H) hNIS-mGFP function quantified by ^99mTcO₄⁻ uptake relative to untransduced control HLCs. The hNIS co-substrate perchlorate served as a ^99mTcO₄⁻ uptake specificity control. N = 3 biological replicates (with three (B, G-H) or four (C-F) technical repeats per biological sample); error bars are SEM (B-F) or SD (G-H). Statistical tests were Students’ t-test (B-F) or one-way ANOVA with Tukey's multiple comparison correction (G-H); “ns” represents p> 0.05.