Fig. 3.

Non-invasive in vivo imaging of hNIS-mGFP⁺ CGT10 HLCs | (A) Scheme depicting the experimental set-up; briefly, ^99mTcO₄⁻-labelled hNIS-mGFP⁺ HLCs were administered to NSG mice, animals imaged, and after radiotracer decay re-imaged post systemic ^99mTcO₄⁻ injection on the next day. (B/left) SPECT/CT maximum intensity projection (MIP) of pre-labelled and intrahepatically administered CGT10 HLC (cf. A/top). (B/right) 3D volume rendering of SPECT signals subsequent to local signal/noise thresholding (Otsu method) and overlaid onto CT image (blue=cell pellet phantoms; gold=in vivo administered cells). (C) SPECT/CT MIP obtained post systemic ^99mTcO₄⁻ injection demonstrating intrahepatic detection of hNIS-mGFP⁺ HLCs 24 h post cell transplantation but cell loss at 7d Endogenous NIS signals correspond to thyroid/salivary glands (T/S) and the stomach (St). (D-E) Naïve animals were SPECT/CT imaged as in C with only endogenous murine NIS signals being evident (St=stomach; T/S=thyroid/salivary glands). (F) Image-derived in vivo biodistribution data (24 h post administration) corresponding to n = 2 naïve control mice and n = 4 HLC mice. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)