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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Biophys Chem. 1998 Jul 13;73(1-2):53–75. doi: 10.1016/s0301-4622(98)00137-9

Table 1.

Acrylamide quenching of NATA in propylene glycol — multi-exponential analysis of NATA fluorescence intensity decays at different temperatures

Temp (°C) [Acrylamide] τi (ns) τ¯(ns) αi fi χR2
1 exp. 2 exp. 3 exp.
20 0 5.277 5.277 1.0 1.0 1.23
0.5 3.138 0.553 0.833
1.261 0.227 0.137
0.281 2.795 0.220 0.030 239.72 2.96 1.07
1.0 2.358 0.238 0.529
1.213 0.360 0.410
0.162 1.755 0.402 0.061 466.04 6.21 0.76
1.5 1.506 0.258 0.685
0.561 0.230 0.228
0.096 1.168 0.512 0.087 909.45 8.69 1.38
0 0 6.356 0.950 0.992
0.936 6.313 0.050 0.008 6.71 0.52
0.505 5.407 0.460 0.715
2.731 0.288 0.242
0.549 4.293 0.252 0.043 245.74 2.33 0.90
1.010 3.891 0.315 0.651
1.820 0.296 0.286
0.304 3.073 0.389 0.063 641.86 5.99 1.02
1.515 2.851 0.247 0.701
0.964 0.244 0.234
0.128 2.232 0.509 0.065 1132.66 13.92 0.83
−20 0 8.519 0.317 0.534
4.953 0.464 0.454
0.277 6.794 −0.219 −0.012 23.65 7.91 0.45
0.512 6.596 0.579 0.769
2.721 5.701 0.421 0.231 65.91 0.74
1.023 7.650 0.154 0.321
4.109 0.509 0.569
1.204 4.962 0.337 0.110 247.87 2.23 0.89
1.535 6.318 0.195 0.465
2.853 0.393 0.423
0.717 4.225 0.412 0.112 587.20 6.68 1.38
−40 0 8.452 0.200 0.578
4.523 0.548 0.394
0.477 5.931 −0.252 −0.028 62.89 10.60 1.07
0.518 6.759 0.343 0.613
3.269 0.437 0.378
0.159 5.378 −0.220 −0.009 22.66 7.32 1.37
1.036 6.094 0.426 0.649
2.439 4.811 0.574 0.351 104.75 1.92
1.554 5.959 0.291 0.573
2.339 0.520 0.402
0.402 4.365 0.189 0.025 272.67 7.53 1.24
−60 0 12.432 0.020 0.045
5.387 5.704 0.980 0.955 2.15 1.22
0.524 6.193 0.385 0.591
3.444 0.445 0.381
0.675 4.991 0.170 0.028 119.07 2.20 0.28
1.048 6.075 0.296 0.582
2.839 0.395 0.362
0.563 4.595 0.309 0.056 412.20 6.55 0.67
1.572 5.761 0.227 0.544
2.431 0.373 0.377
0.471 4.088 0.400 0.079 725.81 11.01 0.82