Skip to main content
. 2018 Dec 25;35(4):405–409. doi: 10.5511/plantbiotechnology.18.0918a

Table 1. Primer sequences used to generate the constructs expressing the prolamin-GFP fusion proteins under the control of other prolamin promoters.

Primer name Upstream and downstream primer sequences
13b-2-GFP T-Nos fw+KpnI 5′-aggggtaccgcattatacagcaaaataga-3′
13a-1-GFP T-Nos fw+XbaI 5′-atatctagagttgaagcatagtagtagaatcc-3′
T-Nos rv+EcoRI 5′-agagaattcccgatctagtaacatagatg-3′
13b-2 prolamin promoter fw+SalI 5′-gtcgactgtccatcattcctaacaagagg-3′
13b-2 prolamin promoter rv+XbaI 5′-atatctagactgtgtgaacgatgaggagg-3′
13a-1 prolamin promoter fw+SalI 5′-aaagtcgacgaggagggtcagctgtggc-3′
13a-1 prolamin promoter rv+KpnI 5′-ataggtacctgtgtgaaggatgaggaggg-3′
10 kDa prolamin promoter fw+HindIII 5′-ataaagcttgacgggatcacgatctggg-3′
10 kDa prolamin promoter fw+SalI 5′-atagtcgacgacgggatcacgatctggg-3′
10 kDa prolamin promoter rv+SalI 5′-atagtcgacggaataatggttgcctaggg-3′