Table 1.
List of in vitro studies and tested parameters
| Year/country | Author | Cells | Cells’s source | Test | Materials | Control | Duration | Risk of bias | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2011 China[16] | Ma et al. | Human gingival fibroblasts | From healthy patients who underwent oral surgery | MTT assay- SEM -EDAX | ERRM Putty -ERRM Paste -GMTA -IRM -Cavit G |
DMEM incubated for 24 h (negative control) | 1, 3, and 7 days | Moderate | |||
| 2011 USA/ Michigan[17] | Damas et al. | Human dermal fibroblasts | Adult human dermal fibroblasts | MTT assay | RRM -RRP Putty -White MTA- Angelus -ProRoot MTA |
-Culture medium (negative control) -culture medium +cholorhexidine (positive control) |
1 day | Low | |||
| 2012 USA/ Michigan[18] | Hirschman et al. | Human dermal fibroblasts | Adult human dermal fibroblasts | MTT assay | MTA Angelus -ERRM -Ultra-blend Plus -Dycal |
-Culture medium (negative control) -Culture medium +chlorhexidine (positive control) |
2, 5, and 8 days | Low | |||
| 2012 Brazil[7] | De-Deus et al. | Human osteoblast | 36 human maxillary incisor teeth | XTT -NR -CVDE |
iRoot BP Pus -White ProRoot MTA |
-Zinc oxide eugenol positive control -Medium not exposed to material negative control |
24 and 48 h | Low | |||
| 2012 Iran[19] | Shokouhinejad et al. | Cement/ dentin-cement | 30 extracted single-rooted human teeth | SEM -EDX assay |
ERRM -Bioaggregate -ProRoot MTA |
1 week and 2 months | Moderate | ||||
| 2012 China[10] | Zhang et al. | hDPCs | Molars of patients who needed orthodontic treatment | -CCK-8 assay -ALP activity -qRT- PCR assays |
iRoot BP Plus - Bioaggregate -MTA |
Cells without material | 1, 3, 5, and 7 days 3, 5, and 7 days | Low | |||
| 2013 Germany[20] | Willershausen et al. | Human periodontal ligament fibroblasts and human osteoblasts | Promocell, Heidelberg, Germany | ERRM -ProRoot MTA -MTA-Angelus GMTA -WMTA |
Almar blue-fluorescence microscope | Cells without material | 6 h, 1, 3, and 4 days 24 h | Low | |||
| 2014 India[21] | Samyuktha et al. | Human periodontal ligament fibroblasts | Healthy premolar extracted for orthodontic treatment | ERRM -MTA -Biodentine |
Trypan blue dye assay -inverted phase contrast microscope |
Alpha MEM culture medium | 1 and 2 days | Low | |||
| 2014 Turkey[8] | Öncel Torun et al. | hDPCs | Molars of 5 patients undergoing orthodontic treatment | iRoot BP -MTA |
XTT assay -qRT-PCR |
Untreated cell cultures | 1, 2, and 3 days | Moderate | |||
| 2014 China[22] | Zhu et al. | Human dental pulp stem cells (DPSCs) | Extracted 3rd molars | iRoot BP Plus ProRoo t MTA | CCK-8 assay -EDU incorporation assay -Scratch wound healing assay -Transwell assay -SEM |
Cells without material | 1,2,3 and 7 days | Low | |||
| 2015 China[23] | Zhang et al. | DPCs | - | -iRoot BP Plus t MTA | -Scratch wound healing -proRoo Transwell assay -Annexin-V propidium iodide |
- | - | - | |||
| 2015 China[9] | Liu et al. | hDPCs | Impacted 3rd molars or premolars extracted for orthodontic treatment | iRoot BP Plus -MTA |
MTT assay | Cells without material | 1, 3, 5, and 7 days | Low | |||
| 2016 Turkey[24] | Torun et al. | Human dental pulp cells (hDPCs) | Extracted teeth of patients who undergoing orthodontic treatment | iRoot BP | Microarray analysis -qRT-PCR |
Cells without material | 1 and 3 days | Moderate | |||
| 2016 Pennsylvania[25] | Chen et al. | -DPSCs -PDLSCs -hBMSCs |
Center of Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA -Thermo Scientific (Rockford, IL) |
RRM -ProRoot MTA |
MTT assay-SEM- immunoblot analysis | Wells with non-coated cover glass | 1, 3, and 5 days -3 days |
Moderate | |||
| 2016 Peru[26] | Coaguila- Llerena et al. | Human periodontal ligament fibroblasts | Erupted premolars extracted for orthodontic treatment | ERRM -MTA Angelus -Super EBA |
MTT assay | Culture medium Negative control |
1, 3, and 7 days | Low | |||
| 2017 Mexico[27] | Martínez- Cortés et al. | Human gingival fibroblasts | Gingival tissues donated from healthy patients | -ERRM -MTA Angelus -IRM |
MTT assay | Culture media | 1 h, 1 and 3 days | Low | |||
| 2017 India[28] | Sultana et al. | Human bone marrow-derived mesenchymal stem cells (hBMSCs) | _ | ERRM -ProRoot MTA -ALP activity -Biodentine -CLSM -GIC |
MTT assay | -GIC negative control hBMSCs positive control | 1, 3, 5, and 7 days -7 and 21 days |
Low | |||
ERRM = EndoSequence root repair material, MTA = mineral trioxide aggregate, GMTA = gray mineral trioxide aggregate, WMTA = white mineral trioxide aggregate, UBP = ultra- blend Plus, GIC = glass–ionomer cement, IRM = intermediate restoration material, hDPCs = human dental pulp cells, DPSCs = dental pulp stem cells, hBMSCs = human bone marrow- derived mesenchymal stem cells, DPCs = dental pulp cells, PDLSCs = periodontal ligament stem cells, MTT = methyl thiazol tetrazolium, NR = neutral red dye, CVDE = Crystal violet dye elution, CCK = cell counting kit, EDU = Scratch wound healing assay Transwell assay Annexin V propidium iodine, ALP = alkaline phosphatase activity, qRT-PCR = quantitative reverse transcriptase polymerase chain reaction, CLSM = confocal laser scanning, EDAX = energy dispersive analysis by X-ray, SEM = scanning electron microscopy